BT-474

BT-474，亦称ATCC HTB-20，是人类高度非整倍体乳腺导管癌细胞系^[1]，最初分离自一位60岁的白人女性乳腺浸润性导管癌患者。BT-474细胞包含着许多具有典型凋亡细胞形态的细胞，已知细胞不存在参与凋亡诱导的p53蛋白，而抑制细胞凋亡的Bcl-2（英语：Bcl-2）则以中等水平表达。BT-474细胞倾向转移到局部淋巴结，并且能够在肺部形成微转移病变^[2]。BT-474有55个染色体，并且具有极其复杂的基因组序列，大多数染色体计数在超四倍体的范围内，同时又缺失三个染色体（N11、N13和N22），并且某些染色体（N9、N14和N15）存在着低于适当比例的问题^[3]。经过吉姆萨胰蛋白酶带的测试后，未显示出有明显的HeLa标记物，并且在放射免疫分析中，没有检测到α-乳清蛋白的产生，但是发现BT-483细胞高度表达着孕酮受体^[4]。BT-474应用于研究癌症生物学和开发新药物^[3]。

参考资料

^ Lasfargues, EY; Coutinho, WG; Dion, AS. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication.. In vitro. 1979-09, 15 (9): 723–9 [2019-12-07]. PMID 94035. doi:10.1007/bf02618252.
^ van Slooten, HJ; Bonsing, BA; Hiller, AJ; Colbern, GT; van Dierendonck, JH; Cornelisse, CJ; Smith, HS. Outgrowth of BT-474 human breast cancer cells in immune-deficient mice: a new in vivo model for hormone-dependent breast cancer.. British journal of cancer. 1995-07, 72 (1): 22–30 [2019-12-07]. PMID 7599056. doi:10.1038/bjc.1995.271.
^ ^3.0 ^3.1 Ciotlos, S; Mao, Q; Zhang, RY; Li, Z; Chin, R; Gulbahce, N; Liu, SJ; Drmanac, R; Peters, BA. Whole genome sequence analysis of BT-474 using complete Genomics' standard and long fragment read technologies.. GigaScience. 2016, 5: 8 [2019-12-07]. PMID 26865974. doi:10.1186/s13742-016-0113-x.
^ Lasfargues, EY; Coutinho, WG; Redfield, ES. Isolation of two human tumor epithelial cell lines from solid breast carcinomas.. Journal of the National Cancer Institute. 1978-10, 61 (4): 967–78 [2019-12-07]. PMID 212572. （原始内容存档于2013-12-17）.

外部链接

Cellosaurus entry for BT-474（页面存档备份，存于互联网档案馆）

[1] Lasfargues, EY; Coutinho, WG; Dion, AS. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication.. In vitro. 1979-09, 15 (9): 723–9 [2019-12-07]. PMID 94035. doi:10.1007/bf02618252.

[2] van Slooten, HJ; Bonsing, BA; Hiller, AJ; Colbern, GT; van Dierendonck, JH; Cornelisse, CJ; Smith, HS. Outgrowth of BT-474 human breast cancer cells in immune-deficient mice: a new in vivo model for hormone-dependent breast cancer.. British journal of cancer. 1995-07, 72 (1): 22–30 [2019-12-07]. PMID 7599056. doi:10.1038/bjc.1995.271.

[a-3] 3.0 ^3.1 Ciotlos, S; Mao, Q; Zhang, RY; Li, Z; Chin, R; Gulbahce, N; Liu, SJ; Drmanac, R; Peters, BA. Whole genome sequence analysis of BT-474 using complete Genomics' standard and long fragment read technologies.. GigaScience. 2016, 5: 8 [2019-12-07]. PMID 26865974. doi:10.1186/s13742-016-0113-x.

[4] Lasfargues, EY; Coutinho, WG; Redfield, ES. Isolation of two human tumor epithelial cell lines from solid breast carcinomas.. Journal of the National Cancer Institute. 1978-10, 61 (4): 967–78 [2019-12-07]. PMID 212572. （原始内容存档于2013-12-17）.

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